Sheng, Shiqi; Yang, Haijun; Song, Yongshun; Chen, Ruoyang; Liang, Shanshan; Fang, Haiping

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

2022, 23,

Sheng, Shiqi; Yang, Haijun; Song, Yongshun; Chen, Ruoyang; Liang, Shanshan; Fang, Haiping

Great efforts have been made to separate micro/nanoparticles in small-volume specimens, but it is a challenge to achieve the simple, maneuverable and low-cost separation of sub-microliter suspension with large separation distances. By simply adding trace amounts of cations (Mg2+/Ca2+/Na+), we experimentally achieved the size-dependent spontaneous separation of colloidal particles in an evaporating droplet with a volume down to 0.2 mu L. The separation distance was at a millimeter level, benefiting the subsequent processing of the specimen. Within only three separating cycles, the mass ratio between particles with diameters of 1.0 mu m and 0.1 mu m can be effectively increased to 13 times of its initial value. A theoretical analysis indicates that this spontaneous separation is attributed to the size-dependent adsorption between the colloidal particles and the aromatic substrate due to the strong hydrated cation-pi interactions.